Isolation and Characterization of a Novel Ochratoxin A- degrading Acinetobacter sp.strain 2-9B

Abstract

Ochratoxin A (OTA) is a polyketide mycotoxin that commonly contaminates agricultural products and causes significant economic losses. In this study, we investigated the OTA degradation ability of Acinetobacter sp. strain 2-9B which was isolated from the soil. The effects of temperature, incubation time, and pH were studied. Furthermore, it was examined whether the washed cell pellet and the culture supernatant of the fermented culture (SFC) of the bacterial cell components could detoxify OTA. The results showed that strain 2-9B, which achieved 100% degradation of 2.5 μg/mL OTA, was identified as Acinetobacter sp. strain 2-9B. It exhibited optimal degradation activity at 2.5 μg/mL under pH 6.0 and 51℃ incubation for 48 hours. The results also suggest that enzymes present in the supernatant of Acinetobacter sp. strain 2-9B were involved in OTA degradation. The biodegradation of OTA is a very feasible method for food and feed purification, as physical and chemical methods may not only eliminate mycotoxins but also nutrients and sensory-related compounds.