Isolation and Characterization of a Novel Autographiviridae Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant Acinetobacter baumannii-Associated Skin and Soft Tissue Infections.

Phitchayapak Wintachai,Kamonnut Singkhamanan,Komwit Surachat

doi:10.3390/v14020194

Phitchayapak Wintachai, Kamonnut Singkhamanan + Show 1 more

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https://doi.org/10.3390/v14020194

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Journal: Viruses	Publication Date: Jan 20, 2022
Citations: 15	License type: CC BY 4.0

Affiliation: Prince of Songkla University, Walailak University

Abstract

Multidrug-resistant Acinetobacter baumannii (MDR A. baumannii) is one of the ESKAPE pathogens that restricts available treatment options. MDR A. baumannii is responsible for a dramatic increase in case numbers of a wide variety of infections, including skin and soft tissue infections (SSTIs), resulting in pyoderma, surgical debridement, and necrotizing fasciitis. To investigate an alternative medical treatment for SSTIs, a broad range lytic Acinetobacter phage, vB _AbP_ABWU2101 (phage vABWU2101), for lysing MDR A. baumannii in associated SSTIs was isolated and the biological aspects of this phage were investigated. Morphological characterization and genomic analysis revealed that phage vABWU2101 was a new species in the Friunavirus, Beijerinckvirinae, family Autographiviridae, and order Caudovirales. Antibiofilm activity of phage vABWU2101 demonstrated good activity against both preformed biofilms and biofilm formation. The combination of phage vABWU2101 and tigecycline showed synergistic antimicrobial activities against planktonic and biofilm cells. Scanning electron microscopy confirmed that the antibacterial efficacy of the combination of phage vABWU2101 and tigecycline was more effective than the phage or antibiotic alone. Hence, our findings could potentially be used to develop a therapeutic option for the treatment of SSTIs caused by MDR A. baumannii.

Highlights

Acinetobacter baumannii (A. baumannii), an opportunistic Gram-negative bacterium, has emerged as a superbug that is a significant multidrug-resistant (MDR) nosocomial pathogen
Recent reports have suggested that MDR A. baumannii is associated with skin and soft tissue infections (SSTIs) [2,3], and A. baumannii infections have been identified as a serious problem in diabetic foot ulcers [4,5]
A. baumannii has been implicated in various infections, including infections of the skin and soft tissues, respiratory infection, meningitis, urinary tract, and sepsis

Summary

Introduction

Acinetobacter baumannii (A. baumannii), an opportunistic Gram-negative bacterium, has emerged as a superbug that is a significant multidrug-resistant (MDR) nosocomial pathogen. The increasing capacity of A. baumannii to develop resistance to multiple antimicrobial agents has been reported continuously, causing significant health problems, an increase in morbidity and mortality, and treatment failures [1]. Recent reports have suggested that MDR A. baumannii is associated with skin and soft tissue infections (SSTIs) [2,3], and A. baumannii infections have been identified as a serious problem in diabetic foot ulcers [4,5]. The induction of MDR A. baumannii infections in SSTIs causes chronic infections, which often contribute to delayed wound healing, pyoderma, necrotizing soft tissue, skin-graft failure, necrotizing fasciitis, and sepsis [7,8]

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