Abstract
A new cyanobacterial strain was isolated and purified from salt Lake Balkhash, Kazakhstan. According to its morphological and ultrastructural characteristics, 16S rRNA sequence and the fatty acid profile, the strain has been classified as Cyanobacterium spp. and assigned as Cyanobacterium sp. IPPAS B-1200. The strain is characterized by a non-temperature inducible Δ9-desaturation system, and by high relative amounts of myristic (14:0—30%) and myristoleic (14:1Δ9—10%) acids. The total amount of C14 fatty acids reaches 40%, which is unusually high for cyanobacteria, and it has never been reported before. The remaining fatty acids are represented mainly by palmitic (16:0) and palmitoleic (16:1Δ9) acids (the sum reaches nearly 60%). Such a fatty acid composition, together with a relatively high speed of growth, makes this newly isolated strain a prospective candidate for biodiesel production.
Highlights
Cyanobacteria are considered as one of the most ancient group of living organismsHow to cite this paper: Sarsekeyeva, F.K., Usserbaeva, A.A., Zayadan, B.K., Mironov, K.S., Sidorov, R.A., Kozlova, A.Yu., Kupriyanova, E.V., Sinetova, M.A. and Los, D.A. (2014) Isolation and Characterization of a New Cyanobacterial Strain with a Unique Fatty Acid Composition
Isolation and Purification of the Cyanobacterial Strain Water samples from Lake Balkhash were plated on BG11 [25] or Zarrouk [17] media
Light (Figure 1) and electron (Figure 2) microscopy of samples revealed the presence of cells of cyanobacteria resembling Cyanobacterium spp. of Chroococcales [27]
Summary
Cyanobacteria (formerly blue-green algae) are considered as one of the most ancient group of living organismsHow to cite this paper: Sarsekeyeva, F.K., Usserbaeva, A.A., Zayadan, B.K., Mironov, K.S., Sidorov, R.A., Kozlova, A.Yu., Kupriyanova, E.V., Sinetova, M.A. and Los, D.A. (2014) Isolation and Characterization of a New Cyanobacterial Strain with a Unique Fatty Acid Composition. Biodiesel is produced by trans-esterification of plant oils, yielding fatty acid methyl or ethyl esters. Such production depends on the availability of vegetable oil feedstock. Cyanobacteria have several advantages in comparison to higher plants and algae: 1) they grow much faster than competitors; 2) they have high photosynthetic efficiency; 3) they are transformable by double homologous recombination. The latter allows targeted mutagenesis and genetic transformation that imply full-scale metabolic design and engineering of organisms that efficiently convert solar energy into high-value products [8]
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