Abstract
In the recent 50 years, marine oil spills had resulted in severe environmental pollution problems worldwide. In this study, 12 petroleum-degrading strains named MJ1 to MJ12, which can use diesel oil as the sole carbon source for growth, were isolated from the seawater in Jiaozhou Bay, China. Strain MJ4 has the highest diesel-degrading rate which is up to 26.54% in 5 days with the diesel oil concentration of 10 g/L. According to the BLAST research, 16SrRNA sequence of MJ4 showed 99% similarity to Bacillus megaterium strain. Single-factor experiments and response surface methodology were carried out to optimize the environmental factors and their reciprocal action for affecting the diesel oil degradation process of Bacillus sp. MJ4. Results of single-factor experiments revealed that the highest degradation rate was obtained with temperature of 28 °C, pH of 8.8, diesel oil concentration of 25 g/L, P/N ratio of 0.56, nitrogen and phosphorus dosage quantity of 0.35 g/L and 0.18 g/L, respectively. A nonlinear regression equation of diesel oil degradation rate and pH, temperature, P/N ratio was obtained. The model predicted the maximum degradation rate of 72.21% with temperature of 28 °C, pH of 8.88, P/N ratio of 0.31, respectively.
Highlights
Nowadays, most of the industries and transport systems still depend on petroleum resource
Oil spills had resulted in severe environmental pollution problems
More than 100 genera, 200 species of petroleum degradation microorganism have been discovered in marine environment, including 79 genera of bacteria, nine genera of cyanobacteria, 103 genera of fungi, and 19 genera of algae (Xue et al 2015)
Summary
Most of the industries and transport systems still depend on petroleum resource. College of Chemical Engineering, China University of Petroleum, Beijing 102249, China that 1.3 million tons of oil has been spilled into the ocean (Bao et al 2012). Since many indigenous microorganisms have the ability to degrade petroleum, microbial technology is considered to be one of the important methods for the remediation of petroleum hydrocarbon pollution (Mahmoudi et al 2013; Xu et al 2020). More than 100 genera, 200 species of petroleum degradation microorganism have been discovered in marine environment, including 79 genera of bacteria, nine genera of cyanobacteria, 103 genera of fungi, and 19 genera of algae (Xue et al 2015). Because of the characters of ocean, environmental factors such as scarcity of necessary nutrients and low temperature limit the growth and degradation abilities of oil-degrading bacteria (Thavasi et al 2011). Dosage of nitrogen and phosphorus should be well investigated before application because they are the main element resulting in eutrophication of water body and structure change of marine ecosystem
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