Isolation and Characterization of a Heparin-Like Compound with Potent Anticoagulant and Fibrinolytic Activity from the Clam Coelomactra antiquata.

Zhenxing Du,Xiaofei Liu,Siyi Zhou,Jianping Chen,Jing Chen,Pengzhi Hong,Xiaohuang Cao,Saiyi Zhong,Xuejing Jia

doi:10.3390/md18010006

Abstract

Heparin from mollusks with unique sulfated glycosaminoglycan exhibits strong anti-thrombotic activities. This study reports on a purified heparinoid from Coelomactra antiquata, which shows potent anticoagulant and fibrinolytic abilities. Its structure was characterized by infrared spectroscopy, high-performance liquid chromatography, and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. Its fibrinolytic activity was determined in vitro and in vivo. Its anticoagulant activity was determined by activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicated that clam heparinoid was a homogeneous glycosaminoglycan with a molecular weight of 30.99 kDa, mainly composed of →4)-α-IdoA2S-(1→4)-α-GlcNS3S6S (or GlcNS6S)-(1→4)-β-GlcA-(1→4)-α-GlcNS6S (or GlcNAC)-(1→. Furthermore, this heparinoid showed a highly anticoagulant titer and fibrinolytic value of 149.63 IU/mg and 1.96 IU/mg, respectively. In summary, clam heparinoid shows great potential for application in the clinic and antithrombotic drugs industry.

Highlights

Heart and vascular diseases, including thrombosis, are the leading causes of death in the UnitedStates and Europe
A natural heparinoid was obtained from the clam C. antiquata by enzymatic hydrolysis, ion
Our results indicated that purified C. antiquata heparinoid with a molecular weight of 30.99 kDa is mainly composed of →4)-α-IdoA2S-(1→4)-α-GlcNS3S6S-(1→4)-β-glucuronic acid (GlcA)

Summary

Introduction

Heart and vascular diseases, including thrombosis, are the leading causes of death in the UnitedStates and Europe. Heart and vascular diseases, including thrombosis, are the leading causes of death in the United. When compared with malignant cancers, they are still the main cause of death. This explains the efforts to discover and develop specific and more potent antithrombotic agents [1]. As a costly and nutrient-rich seafood shellfish, Coelomactra antiquata has attracted a lot of attention in China (Figure 1). According to the Compendium of Materia Medica, C. antiquata has been used as a tonifying essence for lung moistening. Wen et al reported that the use of C. antiquata water extract at doses of 50–150 mg/kg could significantly reduce the blood sugar concentration in

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