Abstract
The isolation and analysis is described of several cDNA- as well as one genomic clone encoding patatin, the major storage protein from tubers of Solanum tuberosum. The structure of the patatin gene was determined by sequencing a near full-length cDNA clone as well as a genomic fragment of 5.3 kb containing the entire RNA coding part plus about 0.8 kb 5′ upstream sequences. The transcription start site was determined by S1-mapping experiments. An open reading frame of 1,158 nucleotides is found in both the cDNA and the genomic clone. Six introns interrupt the ORF in the genomic sequence. This open reading frame allows for a protein of 42 kd which is 2.5 kd larger than the size of the mature protein. The patatin gene has all the characteristic sequences normally found in eukaryotic genes. A TATA box is found 25 nucleotides upstream of the transcription initiation site and the sequence CAAACT at position -60. In the 3′ region the consensus sequence AATAAA is found 13–18 nucleotides in front of the poly-A-addition site.
Published Version
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