Isolation and characterization of a diazotrophic, oxalate-oxidizing bacterium from sour grass ( Oxalis pes-caprae L.)

Abstract

A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 °C, pH 7.5. Growth was observed at 37 and 42 °C with oxalate and in the presence of 3–4% NaCl and 2% potassium oxalate. In liquid culture, the doubling time ( t d ) with oxalate was 9 h. Its closest phylogenetic neighbors, as deduced by 16S rDNA-based analysis, were Azospirillum brasilense, Azospirillum doebereinerae and Azospirillum lipoferum, with 99.5, 98.4 and 96.7% sequence similarity, respectively. The strain differed from A. brasilense by its ability to use N-acetylglucosamine, d-glucose and d-mannitol. It may be a variant strain of A. brasilense. Oxalotrophic, N 2-fixing species of the genus Azospirillum may be important contributors to soil formation, soil fertility, and retention and/or cycling of elements necessary for plant growth.