Isolation and characterization of a cDNA encoding a novel 2-oxoacid-dependent dioxygenase which is up-regulated during adventitious root formation in apple (Malus domestica 'Jork 9') stem discs

Abstract

ODDs typically catalyse such reactions as hydroxylation A cDNA clone encoding a novel 2-oxoacid-dependent di- and epoxidation and are involved in a variety of diVerent oxygenase which is up-regulated during the early phases of biosynthetic pathways (Prescott and John, 1996). The isol- adventitious root formation in Malus domestica 'Jork 9' has ated cDNA has subsequently been designated ARRO-1 been isolated. The corresponding gene has been designated (Adventitious Rooting Related Oxygenase). Sequence com- ARRO-1 (Adventitious Rooting Related Oxygenase) and is parison with other plant dioxygenases revealed that the represented in the apple genome by a number of distinct ARRO-1 cDNA was missing approximately 200 bp from its gene copies. N-terminus. A strategy based on the polymerase chain reac- tion (PCR) was used to amplify the remaining ARRO-1