Isolation and characterization of A cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme

Abstract

1. 1. A mouse lactate dehydrogenase-A cDNA was isolated and it was shown to contain the 393bp of the protein-coding sequence and 488bp of the 3′ untranslated region. 2. 2. The amino acid sequence deduced from its open reading frame provided independent evidence for the sequence of residues 201–331 of mouse LDH-A subunit (muscle). 3. 3. This cDNA clone was used as a probe to isolate a mouse genomic clone containing a truncated, processed LDH-A pseudogene. 4. 4. This pseudogene showed 81.6% homology at 713 positions compared with the LDH-A cDNA sequence. The divergence of this pseudogene was estimated to have occurred 39 million years ago.