Abstract
The black rockfish (Sebastes schlegelii) is an economically important species. However, wild S. schlegelii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of S. schlegelii. In this study, 38 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1400 to 0.6400 and 0.1487 to 0.4978, respectively. The minor allele frequency raged from 0.1429 to 0.4694. Polymorphic information content ranged from 0.174 to 0.365. Four SNPs were found to be deviated significantly from the HWE (P < 0.05). These SNP markers will serve as a useful tool for genetic studies and population evaluation aimed at the conservation of S. schlegelii.
Highlights
The black rockfish (Sebastes schlegelii) is an important commercial fish distributed in Northwest Pacific enjoying high popularity in China, Korea and Japan (Yoshida et al 2005)
38 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing
The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1400 to 0.6400 and 0.1487 to 0.4978, respectively
Summary
The black rockfish (Sebastes schlegelii) is an important commercial fish distributed in Northwest Pacific enjoying high popularity in China, Korea and Japan (Yoshida et al 2005). The black rockfish (Sebastes schlegelii) is an economically important species. 38 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. Polymorphic information content ranged from 0.174 to 0.365.
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