Abstract

We developed 27 polymorphic microsatellite loci by constructing a microsatellite-enriched genomic library. The number of alleles per locus ranged from two to seven, and observed and expected heterozygosities from 0.1905 to 0.8000 and from 0.1765 to 0.8017 respectively. Seven loci (X63, X195, X208, X348, X731, X795, X1214, and XB96) deviated from the Hardy–Weinberg equilibrium in the sampled population after BONFERRONI correction (adjusted P value = 0.003). These microsatellite markers will be useful for the study of population structure, genetic diversity and the construction of the genetic mapping of Macrobrachium nipponense.

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