Isolation and characterization of 25 novel polymorphic microsatellite markers from grass carp (Ctenopharyngodon idella)

Abstract

The grass carp (Ctenopharyngodon idella) is an important, widely distributed freshwater fish species in China. We reported the isolation and characterization of 25 novel polymorphic microsatellite markers from genomic DNA library of grass carp (C. idella) enriched by (CA)10 probe. These loci showed high levels of genetic polymorphism testing on 60 individuals collected from the Heilongjiang River (China). The average allele number was 6.52 per locus, ranging from 3 to 15. The values of observed heterozygosity and expected heterozygosity ranged from 0.350 to 0.950 and from 0.439 to 0.843, respectively. The 22 of the 25 microsatellites conformed to Hardy–Weinberg equilibrium. These polymorphic microsatellite markers can be useful in population genetic studies, conservation strategies and further genetic improvement for this species.