Isolation and characterization of 25 novel EST-SNP markers in grass carp (Ctenopharyngodon idella)

Abstract

The grass carp (Ctenopharyngodon idella) is one of the most important freshwater fish species in China. In this study, we reported the isolation and characterization of 25 novel single nucleotide polymorphism (SNP) markers from 47 wild individuals (collected from Yangtze River). The ranges of observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content were 0.0213–0.5532, 0.0213–0.5059 and 0.0208–0.3749, respectively. Six of the characterized loci were significantly deviated from Hardy–Weinberg equilibrium (P < 0.05). Except for two loci, which were in the same unigene, the rest had no linkage disequilibrium between loci pairs. Those novel EST-SNP markers may be useful for conservation strategies and further genetic improvement of grass carp.