Abstract
To evaluate the population genetics structure as a means of devising conservation strategies, we developed microsatellite primers for Sophora koreensis, a narrowly endemic and endangered species in Korea. Thirteen polymorphic microsatellite markers were developed in Korean populations of S. koreensis. Genetic diversity was analyzed in 40 individuals from two populations. The number of alleles per locus ranged from 4 to 14, with observed and expected heterozygosities ranging from 0.200 to 1.000 and from 0.189 to 0.864, respectively. The microsatellite markers described here are valuable tools for the population genetics research of S. koreensis. They can be used to obtain information for creating suitable management strategies to conserve this endemic and endangered species.
Highlights
Sophora koreensis Nakai (Fabaceae) is a deciduous small shrub
Taxonomic studies based on pollen and petal morphologies and molecular evidence [4,5,6] have found that Echinosophora cannot be so clearly distinguished
We have developed a set of polymorphic and co-dominant microsatellite markers for S. koreensis that can serve as effective genetic markers
Summary
Sophora koreensis Nakai (Fabaceae) is a deciduous small shrub Endemic, it is found at only a few locations in central Korea. Taxonomic studies based on pollen and petal morphologies and molecular evidence [4,5,6] have found that Echinosophora cannot be so clearly distinguished It is treated again as a species within Sophora [7]. A population genetics study using dominant ISSR markers was performed to investigate the clonal and spatial genetic structures of S. koreensis [1]. Those results were limited because the use of dominant markers does not allow scoring of genotypes. By conducting further assessments of its genetic diversity and population structure, we can strive toward establishing a strategy for its conservation
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