Abstract
This work was aimed at isolating and characterizing some bioactive constituent(s) from the stem bark of Ficus abutilifolia . The phytochemical analysis of the crude methanol extract indicates the presence of flavonoids, alkaloids, cardiac glycosides, saponnins, tannin, steroids and triterpenes. The extract was subjected to column chromatography which resulted in the isolation and purification of a white needle-like crystalline substance with a melting point of 134 – 136 0 C, the 13 CNMR shows recognizable peak at 71.98 ppm assignable to the beta hydroxyl group and the 1 HNMR spectrum which shows an olefinic proton at δ 5.37 was identified as β-sitosterol. Identification of the compound was purely by spectral evidence. Keywords: Ficus abutilifolia , methanol extracts, phytochemical analysis, β-sitosterol
Highlights
MATERIALS AND METHODPlant collection and methods: The stem bark of Ficus abutilifolia Miq was collected fresh from Samaru, Zaria in Kaduna State, Nigeria in March, 2017
Nature in general, has provided mankind with a complete store house of remedies to cure most human ailments Yadav and Singh, (2011)
This research is aimed at the isolation and characterization of the bioactive compound(s) from the stem bark of Ficus abutilifolia
Summary
Plant collection and methods: The stem bark of Ficus abutilifolia Miq was collected fresh from Samaru, Zaria in Kaduna State, Nigeria in March, 2017. The plant bark was air-dried under a shade, segregated and pulverized by manual pounding using wooden mortar and pestle. The pulverized plant material was stored in a cool dry place to avoid moisture from the environment. Extraction and isolation: Five hundred gram (500 g) of the pulverized stem bark was extracted by cold maceration using 2 liters of methanol at room temperature for 48 hours. The extract was dried under reduced pressure using the rotatory evaporator at 400C. The crude extract was subjected to the qualitative phytochemicals analysis using standard methods of Harborne, (1998) (Table 1). Phytochemical screening of the extracts indicated the presence of flavonoids,
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