Isolation and characteristics of cellulolytic bacteria based on corn waste as a fibrous feed bioactivator

S Wahyuni S Wahyuni,E Mirwandhono E Mirwandhono,M Tafsin M Tafsin,I Sembiring I Sembiring,Yunilas Yunilas

doi:10.32734/jpi.v6i3.2155

Abstract

Corn waste is an agricultural product that has the potential to be used as animal feed. The aims of this study was to isolate corn waste bacteria and look for the degradation ability of bacterial fiber of corn waste as a fibrous feed bioactivator. This study was conducted in livestock production laboratory of livestock study program in faculty of agriculture, university of north Sumatra, period March to June 2018. This study used corn waste (corncob, corn straw, corn clobot, corn stalks, and corn root). Research methods used pour plate isolate methods. Parameters observed are macroscopic morphology (colony form, colony color, the edge of the colony , and colony elevesi), microscopic morphology (cell shape and gram coloring), and test the ability to degrade fiber in bacteria. The isolation result obtained 8 isolates with varied shapes and there are 6 isolates that have ability to degrade fibers in bacteria include B2, B3, B4, B5, B6, B7. The conclusion of this study were obtained 8 cellulolytic bacteria isolates and B3 has a high potential to degrade the fiber.

Highlights

Corn waste is an agricultural product that has the potential to be used as animal feed
3.1 Isolation of Corn Waste Bacteria In this study, the isolation method used was the isolation method on the media of the pouring plate [8] by taking the diluted sample and the sample which was circulated into the media
In this study 8 isolates grown on selective media were found to be cellulolytic bacteria

Summary

Introduction

Corn waste is an agricultural product that has the potential to be used as animal feed. The waste produced from corn plants is corn straw (stover), corn fruit bark (klobot), and corn cobs which are usually not used anymore or the economic value is very low. The corn waste is the stem 3 ton/ha, corn straw 1,2 tons/ha, corn straw 0,6 tons/ha, corncob 1,2 tons/ha [1]. The use of cellulolytic microorganisme or cellulase enzymes to degrade crude fiber content can be used as an alternative to improve nutrient quality and digestibility of corn waste feed. Indigenous microorganisms are microbes derived from their own substrate which have optimal ability to degrade fibrous feed. Multi enzymes will be produced which are very instrumental in the feed processing process [2]

Objectives

Methods

Results