Abstract
Papain and alcalase were successively used to hydrolyze grass carp scales for preparation of crude polysaccharide. Chondroitin sulfate were further purified by DEAE-Sepharose Fast Flow and Sepharose 6 Fast Flow gel column chromatograph, and indentified by IR, NMR, element analysis and chemical analysis. Three polysaccharide fractions including chondroitin sulfate were fistly obtained from grass carp scales. The weight-average molecular weight of chondroitin sulfate was 46168 Da. The contents of various elements (C, H, N, S, O) in chondroitin sulfate were 28.89 %, 3.80 %, 3.36 %, 6.71 % and 47.16 %, respectively. The results of IR, NMR and chemical analysis exhibited that the sulfate group in chondroitin sulfate be O-linked in position 4 of N-acetyl-D-galactosamine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
