Abstract
Hazel dormice, Muscardinus avellanarius (Rodentia: Gliridae) are vulnerable to habitat loss and fragmentation, and thus protected by European Directives. We isolated hazel dormouse microsatellite sequences from enriched genomic libraries to facilitate conservation- focussed research, such as population genetics, regarding this threatened species. Fifty-three primer sets were designed from 51 newly isolated loci. Additionally, we redesigned and tested nine primer sets from previously- published hazel dormouse microsatellite sequences. These 62 marker sets were initially tested in eight unrelated individuals. Thirty-nine loci, which were polymorphic and amplified in(88 % of these samples (extracted from hair), were then genotyped and characterised in 22-26 individ- uals. Of these, 26 autosomal loci (18 new and eight pub- lished) adhered to Hardy-Weinberg equilibrium (p B 0.05) and displayed an estimated null allele frequency of \0.10. One loci pair displayed linkage disequilibrium after cor- rection for multiple tests.
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