Isolation and characterisation of CD166+/EpCAM+ and CD166+/CD44+as markers for cancer stem cells from non-small cell lung cancer of A549

Abstract

Event Abstract Back to Event Isolation and characterisation of CD166+/EpCAM+ and CD166+/CD44+as markers for cancer stem cells from non-small cell lung cancer of A549 Noor Hanis A. Halim1, Norashikin Zakaria1 and Badrul H. Yahaya1* 1 Universiti Sains Malaysia, Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI) , Malaysia Lung cancer is often incurable and remains the leading cancer killer in both men and women worldwide [1]. Non-small cell lung cancer which can be subdivided into three major histologic subtypes including adenocarcinoma (AC), squamous cell carcinoma (SCC) and large cell carcinoma (LCC) comprises approximately 80% out of total lung cancer incidence [2]. The failure of current treatment to fully eradicate cancer cells suggested the existence of a minority of cancer cells which exhibit similar characteristics as normal stem cells, named as cancer stem cells (CSCs). These rare subpopulations of undifferentiated cells have the unique biological properties necessary for tumour initiation, maintenance and spreading [3]. Thus targeting the CSCs will be beneficial for future cancer treatment. Thus, this study was aimed to isolate and characterise the CSC populations from A549 non-small cell lung cancer cell lines. A549 cell was cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. A549 cells that positive for co-expression of CD166+/CD44+ and CD166+/EpCAM+ were analysed and sorted out with a Moflow XDP cytometer. The sorted lung cancer stem cells then were cultured and expanded in vitro until confluent. Clonogenic and differentiation assay were performed to test the pluripotent capability of sorted CSCs. Both CD166+/EpCAM+ and CD166+/CD44+ showed ability to form colonies and able to differentiate into adipocytes and osteocytes (Fig.1). Pluripotent capability of putative CSCs was further confirmed by expression of self-renewal gene. Expression of transcription factor genes that critically involved in self-renewal of undifferentiated stem cell; SOX-2, NANOG, KLF4 and POU51 were up-regulated in both putative CSCs population when compared to A549 parental. Among these four genes, NANOG showed the highest expression in both populations with 11.71 and 38.05-fold change (FC), respectively (Fig.2). Results obtained in this study showed that both isolated cell populations have pluripotent capability of stem cells. Thus, it was proved that CD166+/EpCAM and CD166+/CD44+ can be used as markers for CSCs for NSCLC of A549. Figure 1 Figure 2 Acknowledgements This study was funded by Fundamental Research Grant Scheme (FRGS) by the Ministry of Higher Education of Malaysia (203.CIPPT.6711509) and Universiti Sains Malaysia (USM) Fellowship. Keywords: lung cancer, cancer stem cells, Cancer stem cell markers, Cells, Cancer Conference: 6th Malaysian Tissue Engineering and Regenerative Medicine Scientific Meeting (6th MTERMS) 2016 and 2nd Malaysian Stem Cell Meeting, Seberang Jaya, Penang, Malaysia, 17 Nov - 18 Nov, 2016. Presentation Type: Poster Topic: Stem cells and Regenerative Medicine Citation: Halim NA, Zakaria N and Yahaya BH (2016). Isolation and characterisation of CD166+/EpCAM+ and CD166+/CD44+as markers for cancer stem cells from non-small cell lung cancer of A549. Front. Bioeng. Biotechnol. Conference Abstract: 6th Malaysian Tissue Engineering and Regenerative Medicine Scientific Meeting (6th MTERMS) 2016 and 2nd Malaysian Stem Cell Meeting. doi: 10.3389/conf.FBIOE.2016.02.00007 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. 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Received: 08 Dec 2016; Published Online: 19 Dec 2016. * Correspondence: Dr. Badrul H Yahaya, Universiti Sains Malaysia, Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Kepala Batas, Pulau Pinang, 13200, Malaysia, badrul@usm.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Noor Hanis A Halim Norashikin Zakaria Badrul H Yahaya Google Noor Hanis A Halim Norashikin Zakaria Badrul H Yahaya Google Scholar Noor Hanis A Halim Norashikin Zakaria Badrul H Yahaya PubMed Noor Hanis A Halim Norashikin Zakaria Badrul H Yahaya Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.