Abstract
A method for preparative-scale isolation of the juvenile hormone from ether extracts of abdomens of adult male Hyalophora cecropia has been developed. The purification procedure consists of five steps: low-temperature precipitation, molecular distillation, thin-layer chromatography (two consecutive seperations), and gas-liquid chromatography. A 1·25 × 10 5-fold purification was achieved; 56 per cent of the extracted activity was recovered. Chromatographic and biological data prove that juvenile hormone is not identical with any compound previously reported to have juvenile hormone activity. The isolated material shows prothoracotropic activity in Hyalophora cecropia, gonadotropic activity in Periplaneta americana, and morphogenetic activity in Galleria mellonella larvae and Tenebrio molitor pupae.
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