Abstract

Twenty-eight bacterial strains capable of utilizing the petroleum were isolated from coastal areas along Jiaozhou Bay, Qingdao, China. The biodegradation of diesel and crude oil in seawater by mixed cultures of hydrocarbon-degrading bacteria was investigated. The mixtures or consortia of bacteria, denoted as Consortium 1 and Consortium 2 consisted of 5 bacterial strains, respectively. Consortium 1 was more efficient degrading diesel oil in seawater compared to consortium 2; Consortium 2 could effectively remove crude oil in seawater after a 30-day incubation period. The degradation process fits to a first order model, and consortium 1 had the highest capability in using diesel oil for its growth than consortium 2.

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