Abstract
Isolation and biochemical characterization of Streptococus pneumoniae from throat swabs and sputum of children and adult in Quetta District
Highlights
When the colonies from Crystal Violet Nalidixic Acid Gentamycin (CVNG) were streaked on MacConkey agar, no growth had appeared
To do away with the lack of authentic data on the etiology of pneumonia infection in Quetta Balochistan, this study aimed at investigating in a prospective study the spectrum and susceptibility ofStreptococcus pneumoniae of pneumonia infection and establishing associations with known clinical and demographic risk factors in population referred to a distinct hospital of Quetta city
Collection of samples Throat swabs and sputum samples were collected from outdoor patients of Bolan Medical Complex (BMC) Hospital and Provincial Sandeman Hospital (PSH) in a pre-sterile pre-labeled, dry, wide-necked, leak proof container, care were taken in order to prevent, spread of infectious organisms, and to avoid contamination the outside of the container
Summary
On selective media; Crystal Violet Nalidixic Acid Gentamycin (CVNG) blood agar all (40) isolates produced small, flat, draughtsman type colonies with greenish alpha hemolysis. Pneumoniae (pneumococcus) is an essential pathogen of human that causes both delicate upper respiratory infections and serious invasive infections like septicaemia, meningitis and pneumonia. The proper management is important for the treatment of pneumonia patients. S. pneumoniae cells are lancet-shaped and singly and in short chains. They are seen as pairs between 0.5 and 1.25 mm in diameter. Colonies from Columbia blood agar were streaked on CVNG agar. Colonies appeared as small, flat, draughtsman type with greenish alpha haemolysis as shown in (Fig. 2)
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