Abstract
Ferrolegoglobin can be reproducibly isolated from legume root nodules by extraction in 3 M ammonium sulfate solution at pH 9 in a nonoxidizing atmosphere. Hydrogen gas stabilizes ferrolegoglobin allowing more than 1 year's storage at temperatures below −10 °C. without conversion to ferrilegoglobin. The rate of autoxidation of ferrolegoglobin decreases with decreasing oxygen partial pressure, decreasing ionic strength, and decreasing temperature. It increases with decreasing pH. On the basis of ferrolegoglobin autoxidation and electrophoretic data, the heme group of native ferrolegoglobin is not located in an exposed position on the surface of the globin. The molecule undergoes irreversible rearrangement during autoxidation in air, subsequently resulting in exposure of the heme group.
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