Isolation and assessment of the beneficial effect of exopolysaccharide-producing PGPR in Triticum aestivum (L.) plants grown under NaCl and Cd -stressed conditions

Abstract

Exopolysaccharide (EPS)-producing beneficial bacteria play a multifaceted role in improving plant growth and adaptive responses against different stressors. In this study, we isolated 25 bacterial strains from pea nodules and were further studied for their sodium chloride (NaCl) and cadmium (Cd) stress tolerance. Based on our results, Rhizobium fabae SR-22 (NCBI Accession number: MG063739.1) showed better tolerance toward salinity and Cd stress and produced a wide range of plant growth-promoting compounds. However, the amount of EPS varies during NaCl and Cd stress. It was important to note that NaCl and Cd beyond the tolerant level, affected the morphology and cellular viability of R. fabae. Interestingly, plant growth-promoting (PGP) substances (indole-3-acetic acid, ammonia, siderophore, and ACC deaminase) released by R. fabae were increased with increasing NaCl concentrations. In contrast, PGP substances were greatly decreased by increasing Cd dosages. Further, the beneficial effect of EPS-producing R. fabae in Triticum aestivum grown in soil treated with different levels of NaCl and Cd was assessed. Inoculation of R. fabae in wheat seedlings grown under higher NaCl and Cd concentrations showed improved growth compared to non-inoculated plants. R. fabae exhibited maximum effect in wheat plants grown under 2% NaCl and increased seed germination (8%), root length (13%), vigor indices (19%), root biomass (20%), chlorophyll-a (31%), total chlorophyll (27%) and carotenoid content. Additionally, R. fabae increased Cd and NaCl tolerance in wheat seedlings and improved their antioxidative responses. Conclusively, this work demonstrated that EPS-producing R. fabae showed a promising role in mitigating salinity and Cd-stress in wheat possibly by reducing salt and HM stress-induced abrasions and growth promotion via inorganic phosphate solubilization, and increased nutrient absorption. In the future, R. fabae equipped with these distinguishing characteristics may be used as effective bio-inoculants/bio-formulations in agriculture to address salinity and HM stress issues.