Isolation and Antimicrobial Activity of Rutin and Its Derivatives from Ruta chalepensis (Rutaceae) Growing in Iraq

Abstract

Rutin (1), rutin 3'-methyl ether (2), and a new flavonol glycoside, 6-hydroxy-rutin 3',7-dimethyl ether (3), were isolated from the methanol extract of the fruits of Ruta chalepensis, collected from Diyala, Iraq. Their structures were elucidated by spectroscopic analyses, including 1D-, 2D-NMR and HRESIMS. Antimicrobial activity of compounds 1-3 was tested against four Gram +ve and Gram –ve bacterial strains, and the only fungal strain, Candida albicans, using the 96-well based resazurin microtitre assay.