Abstract
Sixteen fungal strains isolated from the Erigeron canadensis, one of traditional Chinese medicines used to treat the pathogenic infection and dysentery, were evaluated for their antifungal activities against one human pathogen Candida albicans, and two phytopathogens, Colletotrichum fructicola and Rhizoctonia cerealis. The bioassay results indicated that the ethyl acetate extract of the fermentation broth of these fungal endophytes had stronger antimicrobial activities. Among these endophytic strains, the ethyl acetate extracts of strains NPR003 and NPR005 showed the strongest inhibitory effects and has potential application in the discovery of new antifungal agents. This was the first report on the isolation of endophytic fungi from E. canadensis and evaluation of their antifungal activities.
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Highlights
Fungal infection is an important life-threatening disease, which annually causes the death of millions of people (Xie et al, 2014) and the great loss of crop production (Bennett et al, 2012)
Biological investigation of endophytic fungi from healthy E. canadensis lead to isolation of sixteen fungal strains (NPR001-NPR016), which seven stains NPR001NPR007 were from roots, two strains NPR008-NPR009 from flowers, five strains NPR010-NPR014 from stems, and two strains NPR015-NPR016 from leaves
Two strains NPR003 and NPR005 had a broad spectrum of antifungal activities against all testing pathogens
Summary
Fungal infection is an important life-threatening disease, which annually causes the death of millions of people (Xie et al, 2014) and the great loss of crop production (Bennett et al, 2012). There is an urgent need to search for alternative approaches to accelerate the discovery of more biocontrol agents to treat fungal infections and their drug resistance. A growing evidence indicates that endophyte, a special microbial community colonized in healthy plants, is one of the rich sources of bioactive natural products with great potential application in the fields of medicine and agriculture (Zhang et al, 2006; 2014). Isolation and antifungal evaluation of endophytic microbe would provide a better alternative way to identify novel antifungal agents
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