ISOLATION & FORMULATION OF POMEGRANATE JUICE BASED ON PROBIOTIC PROPERTIES

Abstract

Probiotics are mainly living microorganisms that used to improve or restore gut flora when ingested. The consumption of probiotics is mainly regarded as safe.A strain of lactobacillus bacteria strain Streptococcus faecalis strains were isolated from bifilac probiotic medicine and identified by colony morphologies cultured on MRS (Man, Rogosa and Sharpe) media and biochemical testing were done in this study. Isolated bacteria were chosen for probiotic screening and further investigation was done based on biochemical properties and further screening. In this study, Gram staining, Catalase test, carbohydrate fermentation, protease test, starch hydrolysis test, motility test, urease, catalase, nitrate test, indole test, growth optimization (pH optimization, and temperature optimization) were all used . Based on the OD (Optical Density), isolated bacteria may thrive at pH 9.0 and at room temperature of 25 ℃, making them suitable for use as a probiotic.The shelf life of prepared health drink was also check by checking the growth of bacteria on to the EMB agar media. So, different test revealed that S. faecalis can used as a probiotic and also for the development of probiotic health drink. So Probiotics used in gut microbiota by eliciting an immune response that recognises them as beneficial and forms a protective barrier in the intestine against infections.