ISOLATING THE MONOKARYON COLLECTION OF Pleurotus spp.

Abstract

Pleurotus spp. is one of the most important cultivated mushrooms in the world by their nutrition and medicinal property. In Vietnam, although many Pleurotus species are popularly cultivated, but most of them are imported. These strains are easily degenerated when they are cultivated in large scale, because they are difficult to adapt to the environment of Vietnam. This research aims to construct the monokaryon collection of Pleurotus spp. with details of taxonomy, genetic diversity, cultivating traits, monokaryotic mating types focused on Pleurotus strains with the high yield, genetic stable and adaptability to Vietnam environment. 09 Pleurotus strains were collected in South Vietnam and signed PL1 to PL9. Identification by morphology and phylogenetic analysis based on ITS sequences showed that PL1 is P. citrinopileatus; PL2, PL6 and PL9 are P. ostreatus; PL3 is P. cystidiosus; PL4 and PL8 are P. pulmonarius; PL5 is P. cornucopiae; PL7 is P. incarnatus. AFLP analyses indicated a wide genetic diversity of the collected strains with the similar coefficient 52 – 90 %. Pair of PL2 and PL9 is the most closed genetic distance and pair of PL6 and PL7 is the furthest genetic distance. PL4, PL8 and PL1 adapted well to Vietnam environment. PL2, PL5, PL6 and PL9 are cold strains and formed fruiting bodies slowly. PL3 had the highest biological efficiency, but the longest harvesting period (2.5 – 3 months). Except PL7, the biological efficiencies of other strains are over 50 %. 120 monokaryotic isolates of PL1, PL2 and PL8 are collected and determined the mating type.