Isolating and Identification Of Bacteria Causing Urinary Tract Infections From Hospital Patients And Nursing Staff

Abstract

The current study aimed to isolate and identification the bacteria that cause urinary tract infections from recumbent patients and nursing staff, using traditional and molecular methods. 700 Clinical samples were collected from Azadi Teaching Hospital and Kirkuk Teaching Hospital in Kirkuk city for the period from October 2023 to March 2024 from (500) recumbent patients and 200 nursing staff. 347 (49.6%) of the total samples showed positive results for bacterial growth when cultured on ideal culture media, including mannitol, blood, and MacConkey agars. 353(50.4%) of total samples were appear as negative results for bacterial growth. The findings showed the distribution of bacterial isolates obtained from urine samples of recumbent patients and nursing staff at Azadi Teaching Hospital. Escherichia coli (44.6% and 41.67%) was the most prevalent bacterial species, followed by (13.08% and 13.89%) K. pneumoniae, (11.54% and 11.11%) P. aeruginosa. while, the distribution of bacterial isolates obtained from urine samples of recumbent patients and nursing staff at Kirkuk Teaching Hospital. Escherichia coli (46.9% and 51.85%) was the predominant bacterial species, followed by (16.6% and 14.81%) K. pneumoniae and (9% and 11.11%) P. aeruginosa. for molecular study, 7(50.0%) out of a total of 14 E. coli isolates possessed the gyrA gene. On the other hand, 12(100.0%) isolates out of a total of 14 isolates of K. pneumoniae possess the gyrA gene, while 11(91.7%) isolate out of a total of 12 isolates of P. aeruginosa possesses the gyrA gene. It is concluded that the most common cause of urinary tract infections is Escherichia coli followed by K. pneumoniae and P. aeruginosa, and that one of the most important causes of antibiotic resistance is the presence of the gyrA gene, which causes bacterial resistance to fluoroquinolone.