Abstract
Isolated cardiac tissue allows investigators to study mechanisms underlying normal and pathological conditions, which would otherwise be difficult or impossible to perform invivo. Cultured neonatal rat ventricular cardiac myocytes (NRVM) are widely used to study signaling and growth mechanisms in the heart, primarily due to the versatility, economy, and convenience of this invitro model. However, the lack of a well-defined longitudinal cellular axis greatly hampers the ability to measure contractile function in these cells, and therefore to associate signaling with mechanical function. In these methods, we demonstrate that this limitation can be overcome by using papillary muscles isolated from neonatal rat hearts. In the methods we describe procedures for isolation of right ventricular papillary muscles from 3-day-old neonatal rats and effects of mechanical and humoral stimuli on contraction and relaxation properties of these tissues.
Highlights
The neonatal rat cardiac ventricular myocytes culture (NRVM) is a popular in vitro model (Golden et al 2012) which has been used to study a wide variety of processes such as apoptosis, oxidative stress, hypertrophic growth, signal transduction, and transcriptional regulation (Gerilechaogetu et al 2013; Jenie et al 2013; Lal et al 2013; Lucchese et al 2013; Yu et al 2013; Zhou and Lu 2013)
Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society
The increase in contractile force associated with increasing frequency is thought to reflect cardiac contractile reserve and is reported to be different depending on the range of frequency, age, and species of the animal (Endoh 2004)
Summary
The neonatal rat cardiac ventricular myocytes culture (NRVM) is a popular in vitro model (Golden et al 2012) which has been used to study a wide variety of processes such as apoptosis, oxidative stress, hypertrophic growth, signal transduction, and transcriptional regulation (Gerilechaogetu et al 2013; Jenie et al 2013; Lal et al 2013; Lucchese et al 2013; Yu et al 2013; Zhou and Lu 2013) The utility of these cells has been recently extended by combining isolated NRVM and nonmyocytes with various matrix proteins to form three-dimensional (3-D) scaf-. We describe the isolation procedures and neonatal rat papillary muscle responses to electrical, mechanical, and beta-adrenergic stimuli
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