Isolate the DNA of Musca domestica (Diptera: Muscidae) with Study of the aspect of their Larval and Pupal Behavior

Abstract

Current study included two sections: the first section include collection of samples Musca domestica flies rearing in cages which containing artificial food for nutrition and superfluity it, in order to get to several generations of it, then had selected some samples of larvae and adults for the purpose of extracting DNA, by use the method of Beye and Raeder,1993,this method succeeded to isolate the DNA from larvae, while the method didn’t get through adults because of the speed of growth and cells divisions in the larval stage, as compared with adult . As to The second section is which put dozens of larvae with the wet nutrimental at a home garden and watch the behavior of the larvae to migration looking for pupal stage places, found from the results that most of larvae were left dry nutrition environment look for most the driest places, it were passed distance ranging between 30-40 cm for purpose of the stop and quiet to get ready to enter into pre-pupal stage, and then as to aspect of place of pupae showing results that most larvae preferred to hide under the leaves and plants residues that provide it with a safe place to hide from danger of predators, or devours and camouflage, and then cracks in the soil and the walls.