Abstract
<p><em>The purpose of this study was to isolate</em><em>d</em><em>, select</em><em>ed</em><em> and characterize</em><em>d</em><em> phytase</em><em> </em><em>bacteria derived from volcanic soil in the </em><em>Guci</em><em>, Tegal. Bacteria from the volcanic soil </em><em>was isolated and</em><em> selected on </em><em>Luria </em><em>Betan</em><em>y</em><em> </em><em>(LB) </em><em>media containing </em><em>4</em><em>% phytic acid. </em><em>Selected colonies were </em><em>optimized </em><em>by</em><em> physical character </em><em>with</em><em> </em><em>determining</em><em> the relative activity. Colonies of bacteria could be isolated from the volcanic soil, selected, </em><em>and </em><em>ha</em><em>d </em><em>the highest relative activity in colonies of TG3 and TG3-5. TG3-5 bacterial colonies producing phytate ha</em><em>d</em><em> the highest optimum value at a temperature of 55°C, pH 7, the incubation time of 90 minutes, the substrate concentration of 5% and a best metal cofactor on Zn<sup>2+</sup> (10<sup>-4</sup>M). Volcanic soil in the </em><em>Guci</em><em>, Tegal containing phytase-producing bacteria with certain physical characteristics.A</em><em></em></p>
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