Abstract
Plant genetic engineering technologies enable of introducing insect resistance genes into crop plants. The cry genes, genes encoding for inhibitor of digestive enzymes of a target insect that were isolated from Bacillus thuringiensis can be used for this purpose. Seeds of common bean ( Phaseolus vulgaris ) contain a glycoprotein that inhibits activity of α -amylase of insects. A α -amylase inhibitor was purified from the common bean seeds. The purified α -amylase inhibitor was then fed to cowpea storage weevil, Callosobruchus maculatus . The results showed a lengthened larval development time inside the seed and caused mortality to the insect larvae. This experiment suggests that the α -amylase inhibitor gene from the common bean seed could be used as a candidate gene for genetic engineering of plant resistance to bruchid insects.
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