Abstract
Development of biological potential could to be a solution of pest problems and environmental damage by pesticides. One of the organisms that are currently often be used for biopesticides is the entomopathogenic fungi such as Beauveria brassiana. This study aim to isolate, characterize of pest-resistant fungi and apply it to some agricultural insect pests in vitro. Samples which used were planthoppers, aphids, grasshoppers and isolated fungi Beauveria bassiana from Biogen Laboratory. The research was conducted in Soil and Environmental Biotechnology Laboratory, Dramaga, Bogor. Isolation method with Insect Bait Methode. Isolation and characterization of pest-resistant fungi that are planthoppers and aphids show the result that the great possibilities are the Beauveria bassiana fungus, that is clearly visible from the obtained physical characteristics, the white and sealed hyphae and conidia round oval. In addition, the fungi which used is a pathogenic fungi on the pest of aphids, planthoppers, and grasshoppers.
Highlights
Tumbuhan secara umum maupun pertanian secara khusus tidak selamanya bisa hidup tanpa gangguan
Development of biological potential could to be a solution of pest problems
of the organisms that are currently often be used for biopesticides is the entomopathogenic fungi such
Summary
Satu isolat fungi Beauveria bassiana dari Laboratorium Biogen. Bahan dan Alat Bahan yang digunakan, terdiri dari: kertas saring, aquadest, kertas saring steril, media PDA, natrium hipoklorit 1%, serangga, tissue. Isolasi dan Karakterisasi Beauveria bassiana sebagai Fungi Anti Hama. 3. Serangga yang terinfeksi jamur B. bassiana terlebih dahulu disterilisasi permukaan dengan 1% Natrium hipoklorit selama 3 menit. 5. Serangga kemudian diletakkan dalam petridish berisi tissu lembab steril dan diinkubasikan untuk merangsang pertumbuhan jamur. 6. Spora yang keluar dari tubuhnya kemudian diambil menggunakan ose dan dibiakkan pada Potato Dextrose Agar (PDA) kemudian diinkubasikan selama 7 hari. Uji infeksi fungi anti hama terhadap serangga Fungi anti hama diambilkan 1 ose dan dimasukkan ke dalam media yang sudah diisikan serangga dengan jarak agak jauh, kemudian diinkubasi. Uji positif einfeksi anti hama dilihat jika fungi menutupi tubuh serangga
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