Abstract
Milkfish (Chanos chanos) is an important economic fish that is cultured in brackish water ponds. Milkfish is relatively resistant to diseases that may be caused by associated gut bacteria to improve health and prevent from disease. In the gut of milkfish was screened to obtain potential probiotic bacteria to be developed as probiotic. Some morphological observation such as shape and color of cell and biochemical test such as starch, casein hidrolysis were conducted. Antagonistic test of the potential bacteria isolate was done to Aeromonas hydrophila. This study was conducted from June to September 2017 in Balai Karantina Ikan dan Pengendalian Mutu dan Keamanan Hasil Perikanan Kelas I Medan I Kuala Namu. The result indicated that potential probiotic bacteria in the digestive tract of milkfish were Staphylococcus spp. (UIM01), Micrococcus sadentarius (UIM02), Lactobacillus acidophillus (LIM01) and Micrococcus lylae (LIM02). These bacteria showed to inhibit Aeromonas hydrophila is growth to low extend.
Highlights
an important economic fish that is cultured in brackish water ponds
Milkfish is relatively resistant to diseases
that may be caused by associated gut bacteria to improve health
Summary
Produksi ikan bendeng di Indonesia cukup melimpah, dengan jumlah produksi mencapai 626.878 ton (Direktorat Jenderal Perikanan, 2013). Jenis ikan ini mampu mentolerir salinitas perairan yang luas (0-158 ppt) sehingga digolongkan sebagai ikan euryhaline. Hal ini dapat dilihat dari kebiasaan makan ikan bandeng yang tergolong jenis ikan herbivora yang mempunyai usus yang panjang beberapa kali dari tubuhnya. Pencernaan ikan bandeng terdapat bakteri yang menghasilkan enzim pencernaan yang dapat merombak nutrien makro yang masuk melalui pakan untuk kebutuhan bakteri itu sendiri dan memudahkannya untuk diserap oleh ikan bandeng tersebut (Gatesoupe, 1999). Keberadaan probiotik ini didalam usus inang (baik pada permukaan usus maupun didalam lumen) berperan sebagai pelindung (barrier) terhadap proliferasi (pertumbuhan) patogen, diantaranya melalui mekanisme produksi senyawa yang mampu menghambat pertumbuhan patogen. Berdasarkan uraian tersebut di atas maka akan dilakukan penelitian tentang isolasi dan karakterisasi bakteri probiotik yang berasal dari saluran pencernaan Ikan Bandeng (Chanos chanos) guna meningkatkan produktivitas pada budidaya ikan Bandeng
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