Abstract
The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.
Highlights
The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method
Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and incubated for 48 hours
The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6
Summary
Sebanyak 100 mL sampel nira sawit dikumpulkan langsung dari petani sawit dengan menggunakan botol kaca steril dan dibawa ke laboratorium pada hari yang sama untuk diproses. Sampel kemudian disimpan dalam suhu ruang selama 48 jam untuk fermentasi dari pengenceran 10-7 disebarkan ke permukaan MRS agar di cawan petri dan diinkubasi selama 48 jam pada suhu 37°C dalam kondisi anaerob. Pekerjaan dilakukan pada kondisi steril di dalam laminar air flow. Setelah 48 jam, total koloni BAL dihitung dengan menggunakan rumus : CFU = total koloni BAL x x. Karakteristik Biokimia Sebanyak 6 koloni BAL dipilih secara acak yaitu EHS1, EHS2, EHS3, EHS4, EHS5, dan EHS6. Karakteristik biokimia dilakukan dengan melakukan Gram staining, uji katalase dan tipe fermentasi
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