Abstract
Objective To investigate the effect of isoimperatorin on nasopharyngeal carcinoma CNE2 cell apoptosis and the role of the MAPK/ERK1/2 signaling pathway in inducing apoptosis. Methods Real-time cellular analysis technology (RTCA) and MTT were used to detect cell proliferation; Annexin V-FITC/PI dual-fluorescence flow cytometry analysis, Hoechst 33342 staining, and mitochondrial membrane potential detection kit were used to detect cell apoptosis; western blot was used to detect protein expression. Results Different concentrations of isoimperatorin (10 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20 Conclusion Isoimperatorin can induce nasopharyngeal carcinoma CNE2 cell apoptosis through the MAPK/ERK1/2 signaling pathway.
Highlights
Nasopharyngeal carcinoma (NPC) is among the most common malignant cancer of the head and neck in southern China
Isoimperatorin (ISOIMP) studied in this article is a naturally occurring coumarin class compound and is one of the active ingredients in Changium smyrnioides Wolff, Angelicae dahuricae Radix, Notopterygium incisum, and Glehnia littoralis. It has been found in the previous studies that isoimperatorin has a wide range of pharmacological effects as follows: can promote the differentiation of adipocytes; may prevent diabetes [1]; inhibits the activity of melanocytes; promotes the expression of melanin transport related protein Rab27a; reduces the melanocyte melanin content [2, 3]; reduces the expression of nitric oxide and tumor necrosis factor-α (TNFα) in fibroblast synovial cells [4]; improves the mitochondrial function; protects the acute liver injury induced by carbon tetrachloride [5]; relaxes the blood vessels [6]; and increases the breast cancer cell radiotherapy sensitivity [7]
CNE2 cells were continuously monitored by Real-time cellular analysis technology (RTCA) for at least 72 hours to observe the effects of isoimperatorin treatment on proliferation. e results showed that all tested concentrations of isoimperatorin could inhibit the proliferation of nasopharyngeal carcinoma CNE2 cells after 24 hours of treatment (Figure 1(a))
Summary
To investigate the effect of isoimperatorin on nasopharyngeal carcinoma CNE2 cell apoptosis and the role of the MAPK/ ERK1/2 signaling pathway in inducing apoptosis. The expression levels of proliferation-related protein PCNA as well as antiapoptosis proteins XIAP, survivin, and Bcl-2 were decreased by drug treatment, the expression level of proapoptosis protein Bax was increased, and the expression of the key MAPK/ERK1/2 signaling pathway proteins p-c-Raf, p-MEK, and p-ERK1/2 of were decreased. After activation of the MAPK/ERK1/2 signaling pathway by isoprenaline hydrochloride (ISO), the efficacy of isoimperatorin to downregulate p-c-Raf, p-MEK, and p-ERK1/2 expressions in the MAPK/ERK1/2 signaling pathway and proliferation-related protein PCNA as well as antiapoptosis proteins XIAP, surviving, and Bcl-2 was reduced compared with that of isoimperatorin alone, the effect of upregulating the proapoptotic protein Bax was reduced, and the apoptosis rate was decreased. Isoimperatorin can induce nasopharyngeal carcinoma CNE2 cell apoptosis through the MAPK/ERK1/2 signaling pathway
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