Abstract
Intracavernosal pressure (ICP) is gold standard for the detection of erectile function in animals, but no consensus has yet been achieved on what kind of anesthetic protocol should be applied. A total of 16 adult male Sprague-Dawley rats were randomized into two groups. In group A, chloral hydrate was injected intraperitoneally. Rats in group B were induced in 5% isoflurane for 3 min and then maintained in 1.0–1.5% isoflurane. Mean arterial pressure (MAP), respiratory rate (RR) and heart rate were monitored during all experiments. After ICP detection, tail vein and carotid artery blood were collected. The maximum ICP value, MAP and ICP/MAP ratio in group B was significantly higher than in that of group A. The RR in group A was lower than in that of group B, but the heart rate in group A was higher than in group B. There were no significant differences in both pO2 and pCO2 between groups. While the data showed that animals in group A were relatively hypoxemic. Isoflurane inhalation anesthesia in detection of erectile function could offer a relatively more stable physical state than in that under the effect of chloral hydrate intraperitoneal anesthesia. Isoflurane inhalation anesthesia is more suitable for ICP test.
Highlights
Penile erection is a complex neuro-vasculo-tissue process and can be influenced by many factors, such as diabetes mellitus and cardiovascular diseases1
One rat in group A died of overdose of anesthesia after Intracavernosal pressure (ICP) detection and no arterial blood analysis data was available for that particular rat
ICP is the gold standard for the detection of erectile function in animal experiments, but until now, there is no uniform standard of anesthesia protocols for conducting ICP tests
Summary
Penile erection is a complex neuro-vasculo-tissue process and can be influenced by many factors, such as diabetes mellitus and cardiovascular diseases. International index of erectile function (IIEF) questionnaire, the most popular psychometric test tool, is widely used to assess patients’ erectile function (EF). International index of erectile function (IIEF) questionnaire, the most popular psychometric test tool, is widely used to assess patients’ erectile function (EF)1,4 This questionnaire mainly depends on self-description of patients, and could not be applied to animals. This questionnaire mainly depends on self-description of patients, and could not be applied to animals5 Another method can be used to evaluate erectile function of animal—intracavernosal pressure (ICP). In the present study, we investigated the effect of two frequently established anesthesia protocols (inhalational anesthesia and intraperitoneal anesthesia) on ICP detection. The ultimate aim of this study was to offer scientific evidence as for which anesthesia protocol is more reliable for ICP test
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