Abstract
An HPLC method was used to separate and determine four isoflavone compounds (genistein, daidzein, formononetin, and biochanin A) from different parts (stem, leaf, and flower) of seven common Trifolium species. In general, low isoflavone concentrations were found in T. montanum, T. fragiferum, T. incarnatum, and T. repens, while T. alpestre, T. pratense, and T. subterraneum contained higher amounts of these compounds. Each species and each plant organ have, according to our data, a different distribution of the examined isoflavone compounds. In the flower is the highest amount of genistein (T. subterraneum) daidzein (T. pratense), and biochanin A (T. alpestre). The leaf fractions showed a similar situation in the case of T. subterraneum and T. alpestre but not in the case of T. pratense. The stem fractions have in general the lowest total isoflavone concentrations and are rich in daidzein (T. pratense) or in daidzein and biochanin A (T. subterraneum and T. alpestre).
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