Abstract

Abstract Allozyme variation of eleven heterozymes of nine enzymes was analyzed for fourteen annual brome species in the type section of the genus Bromus with the use of PAGE. Diploids B . japonicus and B. squarrosus had identical zymograms, providing new evidence about their very close genetic relationships. Tetraploids B. secalinus and B . commutatus had very similar enzyme phenotypes, indicating their strong genetic affinity, but still differed in PRX-F homoeozymes of one composite genome. Diploid B. alopecuros appears to be the donor of one genome in the tetraploid B. lanceolatus . Bromus pseudosecalinus could not be one of the diploid progenitors for B . secalinus . 6-PGD gene is duplicated and diverged in several diploids of the section Bromus. All tetraploids studied exhibited different fixed heterozygosities of several heterozymes, suggesting their alloploid nature.

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