Abstract
AbstractIsoelectric focusing was used to study the antibody clonotype patterns of outbred New Zealand White rabbits during pronlonged immunization with the gram positive bacterium Micrococcus lysodeikticus. Antisera were examined by analytical isoelectric focusing in 1% agarose gels using inexpensive carrier ampholytes which were synthesized in the laboratory. Clonotype patterns of antimicrococcal antibodies were visualized by radioautography using [125I]‐labeled micrococcal carbohydrate antigen. Examination of the antibody clonotype patterns in antisera from a number of outbred rabbits revealed that variations in clonotype expression often occurred within an individual during successive rounds of immunization. These studies demonstrate the utility of analytical isoelectric focusing for monitoring antibody clonotype changes within an individual during immunization.
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