Abstract
Chlamydomonas reinhardii 104 mutant strain cells contain many NAD(P)H-cytochrome c reductase activities unrelated to nitrate reduction in addition to an NAD(P)H-cytochrome c reductase related to NAD(P)H-nitrate reductase, which have molecular weights from 37000 to 124000. By combining chromatographic procedures and preparative isoelectric focusing, three major fractions of NAD(P)H-cytochrome c reductase were isolated with pi at 7.1, 5.5 and 5.05, respectively. Of these fractions, only the last one was identified as the diaphorase subunit of the NAD(P)H-nitrate reductase by its ability to reconstitute in vitro the nitrate reductase native complex upon complementation with active terminal nitrate reductase of 305 mutant of C. reinhardii.
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