Abstract
The study presents a new way of analysis of IgG response to staphylococcal antigens. The method is based on the isoelectrofocusing of human immunoglobulins and after blotting, their reactivity with purified staphylococcal antigens: alpha-toxin, lipase and teichoic acid. The method analyses not only total IgG but also the ‘monoclonal’ levels of IgG subclasses (clones based on the isoelectric points of immunoglobulins). When the pattern of IgG response to particular antigens were compared, a great diversity between patients' sera samples was observed. The qualitative and quantitative assessment of sera IgG fractions differentiated by pH gradient revealed the individual character for each patient. No correlation could be observed between IgG pattern and the type of staphylococcal infection. Analysing the subclass of IgG showed that for protein antigens (alpha-toxin, lipase) it was mainly IgG1 but for carbohydrate antigens (teichoic acid) it was IgG2. No traces of IgG3 and IgG4 fractions were observed.
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