Abstract
Anticonvective stabilization of the pH gradient in isoelectric focusing by layers of granulated gels is described. Analytical separations of an artificial mixture of proteins by thin-layer isoelectric focusing have been compared with those obtained by the density gradient technique. Detection of proteins by staining with different dyes and some implications due to the presence of carrier ampholytes are commented. The effect of isoelectric precipitation, electroosmosis and concentration of carrier ampholytes on thin-layer isoelectric focusing has been studied and the load capacity of layers of granulated gels has been determined. The principle of localized spreading with mixtures of the pH 3–10 and narrow pH range carrier ampholytes is described. Different granulated gels of the Sephadex and Bio-Gel series have been compared. In experiments with gels of varying fractionation ranges, gel porosity did not affect the isoelectric points of selected pH marker proteins. Constant isoelectric points of pH marker proteins have been determined under strongly varying experimental conditions of time, voltage and site of application. The potential of the method for different fields of application is illustrated by focusing of serum proteins (α-globulins), enzymes (ribonuclease and deoxyribonuclease) and a snake venom.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
