Isodifferential Derivative Spectrophotometric Assay of the Carbamate Pesticide Carbaryl and its Metabolite in Biological Fluids and Commercial Formulations

Abstract

Abstract Two types of applications (biological fluids and commercial formulations) have been used to demonstrate the advantages of UV derivative spectroscopy using the isodifferential assay for the quantitative simultaneous determination of the insecticide carbaryl and its metabolite 1-naphthol. By a careful selection of the working wavelengths based on the graphical model to measure derivative amplitudes, it is possible to determine both compounds in the range of 0.2–40.0μg/ml in presence of five and ten-fold concentration excess, respectively. Accurate and reproducible results are obtained with no preliminary separation step required.