Isocoumarin derivatives and monoterpene glycoside from the seeds of Orychophragmus violaceus

Abstract

Five new compounds, including four new isocoumarin derivatives [orychophramarin A–D (1–4)] and a new monoterpene glycoside [orychovioside A (5)], together with fourteen known compounds were isolated from the seeds of Orychophragmus violaceus. Their structures were established on the basis of chemical evidence and spectroscopic analysis. Compound 1 showed significant cytotoxicity against HCT-116 and Hela cell lines compared with the positive control group (Cisplatin) with IC50 values of 5.10 and 8.91μM, respectively. Compound 1 exhibited evident cell cycle retardation that arrested HCT-116 cells at G2 phase and induced apoptosis in HCT-116 cells in the further mechanism study.