Abstract
Background Type 2 diabetes mellitus is a serious public health problem worldwide. Accumulating evidence has shown that β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on Methods Glucose-stimulated insulin secretion (GSIS) from Min6 cells was examined by estimating the insulin levels in response to high glucose challenge after culture with ISC supernatant or exogenous Wnt5a. Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) analyses were used to observe changes in the β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on Results We observed a significant increase in insulin secretion from Min6 cells cocultured in vitro with supernatant from db/m mouse ISCs compared to that from Min6 cells cocultured with supernatant from db/db mouse ISCs; The intracellular Ca2+ concentration in Min6 cells increased in cultured in vitro with supernatant from db/m mouse ISCs and exogenous Wnt5a compared to that from control Min6 cells. Culture of Min6 cells with exogenous Wnt5a caused a significant increase in pCamKII, pFoxO1, PDX-1, and Glut2 levels compared to those in Min6 cells cultured alone; this treatment further decreased Ror2 and Cask expression but did not affect β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on Conclusion ISCs regulate insulin secretion from Min6 cells through the Wnt5a protein-induced Wnt-calcium and FoxO1-PDX1-GLUT2-insulin signalling cascades.
Highlights
Type 2 diabetes mellitus (T2DM) is a common metabolic disorder that is currently a serious public health problem
Dysfunction of β-cells leads to a decrease in insulin secretion, which plays an important role in the development of diabetes [1]. e effect of newly discovered cells such as stellate cells on β-cell insulin secretion in the islet microenvironment has been a popular focus of islet function research
These data demonstrated that the Wnt5a protein plays an important role in islet morphology and functional maintenance
Summary
Type 2 diabetes mellitus is a serious public health problem worldwide. Accumulating evidence has shown that β-cell dysfunction is an important mechanism underlying diabetes mellitus. e changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on β cell function play an important role in the development of diabetes. is study aimed at elucidating the mechanism by which ISCs regulate insulin secretion from Min cells via the Wnt5a protein. Accumulating evidence has shown that β-cell dysfunction is an important mechanism underlying diabetes mellitus. Is study aimed at elucidating the mechanism by which ISCs regulate insulin secretion from Min cells via the Wnt5a protein. Glucosestimulated insulin secretion (GSIS) from Min cells was examined by estimating the insulin levels in response to high glucose challenge after culture with ISC supernatant or exogenous Wnt5a. We observed a significant increase in insulin secretion from Min cells cocultured in vitro with supernatant from db/m mouse ISCs compared to that from Min cells cocultured with supernatant from db/db mouse ISCs; e intracellular Ca2+ concentration in Min cells increased in cultured in vitro with supernatant from db/m mouse ISCs and exogenous Wnt5a compared to that from control Min cells. ISCs regulate insulin secretion from Min cells through the Wnt5a protein-induced Wntcalcium and FoxO1-PDX1-GLUT2-insulin signalling cascades
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