Abstract
Abstract: This paper describes a method for pig islet isolation with the following original features: first, we set up a reproducible, rapid, sterile, and simple method for pancreas procurement and preparation with double duct incannulation. Second, we demonstrated that UW solution can be used for pancreas digestion; third, we designed an original method for pancreas digestion, derived from the method originally described by Ricordi, except that a double digestion circuit made it possible to digest continuously, within one step, the pancreas, which makes the method operator‐independent; finally we designed a simple method for islet purification based on a two‐layer Histopaque/ UW density gradient and the use of the COBE 2991 cell processor.
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