Islet Amyloid Polypeptide and Calcitonin Gene-related Peptide Immunoreactivity in Amyloid and Tumor Cells of Canine Pancreatic Endocrine Tumors

Abstract

Seven canine pancreatic endocrine tumors were evaluated immunohistochemically for the presence of islet amyloid polypeptide (IAPP), calcitonin gene-related peptide (CGRP), and several other pancreatic hormones. Four tumors contained cells with IAPP immunoreactivity, and four had cells with CGRP immunoreactivity. IAPP and CGRP immunoreactivities were localized to distinctly different cell populations in three tumors in which both peptides were detected; however, IAPP immunoreactivity consistently was found in cells that also contained insulin immunoreactivity. Amyloid deposits, which were found in three of the seven tumors, showed strong immunoreactivity with antiserum against IAPP and weaker immunoreactivity with antiserum to CGRP. These results indicate that amyloid deposits in canine pancreatic endocrine tumors are likely derived from IAPP, as is amyloid found in human pancreatic endocrine tumors and pancreatic islet amyloid in aged and diabetic persons and cats.