Ishige okamurae reduces blood glucose levels in high-fat diet mice and improves glucose metabolism in the skeletal muscle and pancreas

Hye-Won Yang,You-Jin Jeon,Seyeon Oh,Bo Mi Ryu,Junwon Choi,Myeongjoo Son,Kyunghee Byun

doi:10.1186/s41240-020-00168-5

Hye-Won Yang, You-Jin Jeon + Show 5 more

Open Access

https://doi.org/10.1186/s41240-020-00168-5

Copy DOI

Abstract

Brown alga (Ishige okamurae; IO) dietary supplements have been reported to possess anti-diabetic properties. However, the effects of IO supplements have not been evaluated on glucose metabolism in the pancreas and skeletal muscle. C57BL/6 N male mice (age, 7 weeks) were arranged in five groups: a chow diet with 0.9% saline (NFD/saline group), high-fat diet (HFD) with 0.9% saline (HFD/saline group). high-fat diet with 25 mg/kg IO extract (HFD/25/IOE). high-fat diet with 50 mg/kg IO extract (HFD/50/IOE), and high-fat diet with 75 mg/kg IO extract (HFD/75/IOE). After 4 weeks, the plasma, pancreas, and skeletal muscle samples were collected for biochemical analyses. IOE significantly ameliorated glucose tolerance impairment and fasting and 2 h blood glucose level in HFD mice. IOE also stimulated the protein expressions of the glucose transporters (GLUTs) including GLUT2 and GLUT4 and those of their related transcription factors in the pancreases and skeletal muscles of HFD mice, enhanced glucose metabolism, and regulated blood glucose level. Our results suggest Ishige okamurae extract may reduce blood glucose levels by improving glucose metabolism in the pancreas and skeletal muscle in HFD-induced diabetes.

Highlights

Type 2 diabetes is the complex metabolic disorders characterized by an abnormal glucose metabolism arising from pancreatic β-cell dysfunction and glucose intolerance (Hamza et al 2010, Guilherme et al 2008, Bell et al 2001)
Effect of IO extract (IOE) on glucose tolerance in high-fat diet (HFD) mice To determine whether IOE reduces elevated blood glucose level in HFD mice, we investigated glucose tolerance in HFD mice
We examined the improvement of IOE on glucose metabolism in the pancreas and muscle tissue of HFD mice in controlling blood glucose levels

Summary

Introduction

Type 2 diabetes is the complex metabolic disorders characterized by an abnormal glucose metabolism arising from pancreatic β-cell dysfunction and glucose intolerance (Hamza et al 2010, Guilherme et al 2008, Bell et al 2001). Ishige okamurae (IO), as an edible brown seaweed, has been shown to contain biologically active substances like diphlorethohydroxycarmalol (DPHC), ishophloroglucin A, and fucoxanthin and associated secondary metabolites (Sanjeewa et al 2017). It was shown in a previous study that an ethanolic IO extract (IOE) regulated blood glucose level by increasing the level of glucosemetabolizing enzyme in the liver and promoting insulin resistance in db/db mice which is a leptin receptordeficient model (Min et al 2011). We evaluated the effects of an IOE on the protein levels of glucose GLUT2 and GLUT4 and on those of linked transcription factors of the pancreas and skeletal muscle and on blood glucose levels in HFD mice

Methods

Results

Conclusion